Cat. No.
MAK-0087
Product Name
Rat IgA
Storage
4°C
Species
Rat
Instructions
The rat IgA ELISA kit is designed for measurement of IgA in serum, plasma, and other fluids.
Introduction
The rat IgA ELISA kit is designed for measurement of IgA in serum, plasma, and other fluids. The assay uses goat anti-rat IgA for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-rat IgA antibodies for detection. Both capture and detection antibodies were cross absorbed on rat IgG and IgM agarose columns, thereby ensuring specificity for IgA. Cross-reactivity with immunoglobulins from other species has not been investigated. IgA is present in rat serum at concentrations of 0.1 to 1 mg/ml depending on age and nutritional status.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside rat IgA standards. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. IgA molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of IgA is proportional to the optical density of the test sample and is derived from a standard curve.
