Cat. No.
MAK-0088
Product Name
Rat IgE
Storage
4°C
Species
Rat
Instructions
The rat IgE ELISA kit is designed for the measurement of IgE in serum and plasma.
Introduction
IgE is the least abundant immunoglobulin in mammalian serum. In rats, basal IgE levels vary with strain, ranging from approximately 0.1 to 3 µg/ml, and can increase 20-100-fold in response to allergens or infection.1,2 Maximal responses are observed approximately 25-40 days after immunologic stimulus.
Principle Of The Assay
The rat IgE ELISA kit is designed for the measurement of IgE in serum and plasma. The assay uses goat anti-rat IgE for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-rat IgE antibodies for detection.
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared rat IgE standards. The microtiter wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. IgE molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies. TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgE is proportional to the optical density of the test sample and is derived from a standard curve.
