Cat. No.
MAK-0132
Product Name
Human IgG
Storage
2-8°C
Species
Human
Instructions
This kit allows measurement of human IgG1-4 subclasses1 in serum of mice, rats, dogs, and monkeys.
Introduction
Pharmacokinetic (PK) characterization of human IgG monoclonal antibodies requires measurement of human IgG in serum of species used in preclinical research. This kit allows measurement of human IgG1-4 subclasses in serum of mice, rats, dogs, and monkeys.
Principle Of The Assay
The human IgG ELISA uses affinity purified goat anti-human IgG for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-human IgG for detection. Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside human IgG standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgG molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB reagent is added and incubated for 20 minutes. This results in the development of a blue color. Color development is stopped by the addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of IgG is proportional to the absorbance of the test sample and is derived from a standard curve.
