Cat. No.
MAK-0133
Product Name
Human IgE
Storage
2-8°C
Species
Human
Instructions
The human IgE ELISA kit is for measurement of IgE in human serum and fluids.
Introduction
The human IgE ELISA kit is for measurement of IgE in human serum and fluids.
Principle Of The Assay
Diluted serum samples and standards are incubated in the microtiter wells for one hour with HRP conjugate. If IgE molecules are present, they are sandwiched between the immobilization and detection antibodies. The wells are then washed. TMB reagent is added and incubated for 20 minutes. This results in the development of a blue color if IgE is present. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentrations of IgE in samples are proportional to absorbance and are derived from a standard curve.
