Cat. No.
MABL-024
Application
FSEC, WB
Isotype
Engineer antibody
Species Reactivity
Aquifex aeolicus (strain VF5)
Clone No.
2B12
From
Recombinant Antibody
Specificity
/
Alternative Names
Bacterial Leucine Transporter
UniProt
O67854
Immunogen
Purified LeuT in detergent.
Application Notes
The antibody binds specifically to LeuT is a bacterial symporter specific for small hydrophobic amino acids such as leucine and alanine, driven by the sodium electrochemical gradient across the membrane. The antibody is specific for the outward-open conformation (substrate-free/ sodium- bound states) of LeuT, which is stabilised by mutating Tyr108 to Phe in TM3 (Y108F_LeuT). The 2B12 antibody was selected to bind to this conformational state by fluorescence-detection size-exclusion chromatography (FSEC) and western Blot. The Fab fragment of the antibody was obtained by papain digestion. LeuT is a bacterial symporter specific for small hydrophobic amino acids such as leucine and alanine, driven by the sodium electrochemical gradient across the membrane. Due to the high homology of LeuT to human neurotransmitter transporters, its crystal structure can reveal the mechanism of action of many drugs, such as antidepressants.
Antibody First Published
Krishnamurthy et al. X-ray structures of LeuT in substrate-free outward- open and apo inward-open states. Nature. 2012 Jan 9;481(7382):469-74. PMID:22230955
Note on publication
Describes the role of neurotransmitter sodium symporters (NSS) in chemical neurotransmission, and the importance of LeuT, a bacterial NSS homologue, as a structural template. Crystal structures of the outward-open and inward-open states of LeuT, stabilised by the monoclonal antibodies 2B12 and 6A10, are presented.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
