Cat. No.
MABL-026
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
45666
From
Recombinant Antibody
Specificity
/
Alternative Names
Beta-secretase 2; Aspartic-like protease; Aspartyl protease 1; Asp1; Beta- site amyloid precursor protein cleaving enzyme 2; Down syndrome aspartic protease; DRAP; Memapsin-1; Membrane-associated aspartic protease 1; theta-secretase
UniProt
Q9Y5Z0
Immunogen
Human BACE2 244–333.
Application Notes
The antibody binds specifically to BACE2, an enzyme responsible for the proteolytic cleavage of amyloid precursor protein (APP). The protein is cleaved between residues 690 and 691, leading to the formation of beta-cleaved soluble APP and a C-terminal membrane bound fragment (CTFβ). The latter is then cleaved by gamma-secretase to generate amyloid beta peptide. BACE is the rate-limiting enzyme in the pathway, and is a potential target in the treatment of Alzheimer's Disease (AD),a neurodegenerative disorder characterised by amyloid beta plaques within the brain. The BACE2 protein is encoded in chromosome 21, in a region critical for the development of Down's Syndrome, and may play a role in the amyloid beta deposition in the disease.
Antibody First Published
Banner DW, Gsell B, Benz J, Bertschinger J, Burger D, Brack S, Cuppuleri S, Debulpaep M, Gast A, Grabulovski D, Hennig M, Hilpert H, Huber W, Kuglstatter A, Kusznir E, Laeremans T, Matile H, Miscenic C, Rufer AC, Schlatter D, Steyaert J, Stihle M, Thoma R Mapping the conformational space accessible to BACE2 using surface mutants and cocrystals with Fab fragments, Fynomers and Xaperones.Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):1124-37 PMID:23695257
Note on publication
Describes the role of BACE2 as a potential therapeutic target for the pathogenesis of diabetes and Alzheimer's Disease. Also describes the characterization of the crystal structure of BACE2 with the aid of BACE2-binding antibody Fab fragments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
