Cat. No.
MABL-030
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Clostridium botulinum
Clone No.
MCS6-27
From
Recombinant Antibody
Specificity
/
Alternative Names
BBoNT/B
UniProt
Immunogen
Recombinant BoNT/B fragments expressed in E. coli as GST-fusion proteins.
Application Notes
The antibody binds specifically to BoNT/B, a two-chain neurotoxic protein produced by the bacterium Clostridium botulinum. Botulism, an infection of the bacterium, which is commonly spread through contaminated food, can be a fatal disease, and symptoms include a gradual flaccid paralysis.Paralysis is caused by the internalization by endocytosis of the toxin in specific axons, mediated by the attachment of the heavy chain of the protein to axon surface. Once inside the axon, BoNT/B acts by cleaving SNARE proteins, such as VAMP, which are involved in the docking and fusion of neurosecretory vesicles. This prevents the release of the neurotransmitter acetylcholine, resulting in paralysis of the muscles. The monoclonal antibody MCS6-27 binds to the Hc and H5 fragments of BoNT/B, and the binding epitope is located in the heavy chain, between amino acids E859 and E1291. The monoclonal antibody is able to capture BoNT/B at SDS concentrations between 0 and 0.4 mM, and can be used to screen food samples for botulinum toxin.
Antibody First Published
Scotcher MC, Cheng LW, Stanker LH. Detection of botulinum neurotoxin serotype B at sub mouse LD(50) levels by a sandwich immunoassay and its application to toxin detection in milk. PLoS One. 2010 Jun 10;5(6). PMID:20548779
Note on publication
Describes the characterization of monoclonal antibodies against BoNT/B, and the generation of a sensitive sandwich immunoassay.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
