Cat. No.
MABL-039
Application
Depletion, RIA, TLC, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
7A4
From
Recombinant Antibody
Specificity
7A4 binds to GD2: GD2+ neuroblastoma cell lines and melanomas, small-cell lung carcinomas, rhabdomyosarcomas, Ewings sarcomas, Wilm's tumors and retinoblastomas also expressing GD2. GD2- cell lines or GD2- peripheral blood cells are not bound by the antibody. The antibody and the scFv cross-react weakly with GD3 and GM2. GD2 is a target for tumor therapy due to its high level of expression in the majority of neuroblastoma and melanoma cells, and poor levels of expression in normal tissues.
Alternative Names
GD2
UniProt
Immunogen
7A4 was generated by hyperimmunizing mice with recombinant gamma-interferon-treated LAN-1 neuroblastoma cells.
Application Notes
7A4 is able to lyse tumour cells by complement-mediated cytotoxicity (lytic dose ~10 ng) and can perform in vitro direct binding of labelled mAb to living and glutaraldehyde-fixed cells. Shows strong binding to all neuroblastomas tested in addition to other GD2-expressing cells such as melanomas, small-cell lung carcinomas, rhabdomyosarcomas, Ewings sarcomas, Wilm's tumors and retinoblastomas.TLC analysis can be used to confirm the antibody target antigen. The 7A4 scFv possesses the same specificity as the full-length antibody but slightly weaker binding affinity. The scFv has been used in IF studies (PMID: 9309424).
Antibody First Published
Gross et al. New anti-GD2 monoclonal antibodies produced from gamma- interferon-treated neuroblastoma cells International journal of cancer, 15 April 1989, Vol.43(4), pp.665-71 PMID:2467885
Note on publication
Describes the characterization of 7A4 and other anti-neuroblastoma antibodies by TLC, IHC, in vitro direct binding, complement-cytotoxicity assays and solid phase RIA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
