Cat. No.
MABL-038
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
South-American rattlesnake Crotalus durissus terrificus
Clone No.
CTX-scFv8
From
Recombinant Antibody
Specificity
scFv binds to crotoxin and recognises epitopes overlapping BI-a (an epitopic region at the interface between the CA and CB subunits of the heterodimer) and BIII (an epitopic region on CB involved in the enzymatic activity of CB) - scFv 8 may be able to caused the two subunits to dissociate and suppress the synergistic activity between them. Crotoxin is a phospholipase A2 neurotoxin and is the main toxic component in venome from Crotalus durissus terrificus rattlesnakes, acting by blocking neuromuscular transmission.
Alternative Names
Phospholipase A2 crotoxin basic chain; CB; svPLA2; Cdcum6;Phosphatidylcholine 2-acylhydrolase
UniProt
P24027
Immunogen
8 was constructed from a phage display library using V-genes from 43 non-immunized donors.
Application Notes
scFv 8 is able to reduce the myotoxicity induced by crotoxin and is able to neutralize the lethality induced by the toxin. This scFv was also used for (competitive ELISAs).
Antibody First Published
Cardoso et al. Neutralizing human anti crotoxin scFv isolated from a nonimmunized phage library. Scand J Immunol. 2000 Apr;51(4):337-44. PMID:10736105
Note on publication
Describes the construction and a phage library using V-genes from non-immunizzed donors. Studies were conducted which determined the binding region of the scFv and the neutralizing effect of the scFv on mycotoxicity and lethality in mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
