Cat. No.
MABL-037
Application
crystallization, IP, ELISA
Isotype
Engineer antibody
Species Reactivity
Sheep
Clone No.
VRQ14
From
Recombinant Antibody
Specificity
VRW14 binds to both PrPC (host prion protein) and to PrPSc (pathogenic isoform of the prion protein) - the epitope of the antibody mainly consists of PrP residues 188–199 (C-terminal part of helix H2 and N-terminal part of the H2–H3 loop). Misfolding and aggregation of mutated protein PrP leads to prion diseases which are associated with the conversion of the α-helix rich prion protein (PrPC) into a β-structure- rich insoluble conformer (PrPSc). Prion diseases are neurodegenerative pathologies which affect numerous mammalian species and different mutations in the PRNP gene result in protein products with different susceptibilities to causing disease.
Alternative Names
CD230; PrP scrapie-senstive variant VRQ; PrP; major prion protein
UniProt
P23907
Immunogen
The VRQ14 antibody was prepared by immunizing Prnp0/0 mice with the C-terminal domain of recombinant ovine VRQ variant prion protein. The Fab fragemnt was produced by digestion with papain.
Application Notes
VRQ14 can be used to facilitate the crystallisation of ovine PrP in addition to being used in (sandwich) ELISA and IP studies.
Antibody First Published
Eghiaian et al. Insight into the PrPC-->PrPSc conversion from the structures of antibody-bound ovine prion scrapie-susceptibility variants. Proc Natl Acad Sci U S A. 2004 Jul 13;101(28):10254-9. Epub 2004 Jul 6. PMID:15240887
Note on publication
Describes the use of VRQ14 in helping to deduce the PrPC to PrPSc transition and to aid the crystallisation of PrP.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
