Cat. No.
MABL-043
Application
WB, Block, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
Ig 30
From
Recombinant Antibody
Specificity
This antibody recognises murine and rat β-NGF. It does not cross-react with BSA, aprotinin, EGF and insulin.
Alternative Names
b-NGF; β nerve growth factor; beta nerve growth factor; IgG 30; b-NGF
UniProt
Immunogen
This antibody was raised by immunising rats with polymerised β-NGF.
Application Notes
This antibody specifically recognises native β-NGF in ELISA experiments, and denatured β-NGF in Western blotting (Kenigsberg et al, 1991). It has also been used to detect NGF immunoreactive sites in formaldehyde-fixed tissue (Kenigsberg et al, 1991). This antibody has been shown to block the biological activity associated with NGF, including the outgrowth of NGF-induced processes from rat pheochromocytoma cells, and the increase in choline acetyltransferase activity triggered by NGF (Kenigsberg et al, 1991).
Antibody First Published
Kenigsberg et al. Two distinct monoclonal antibodies raised against mouse beta nerve growth factor. Generation of bi-specific anti-nerve growth factor anti-horseradish peroxidase antibodies for use in a homogeneous enzyme immunoassay. J Immunol Methods. 1991 Feb 15;136(2):247- 57. PMID:1999653
Note on publication
Describes the original generation of this antibody, and its characterisation in ELISA, WB and IHC experiments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
