Cat. No.
MABL-045
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MS-C2
From
Recombinant Antibody
Specificity
This antibody has been shown to bind to NF-L (neurofilament light) and protocadherin PCDHGC3.
Alternative Names
PCDHGC3; NF-L; Neurofilament light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein; Protocadherin gamma-C3; Protocadherin gamma subfamily C 3, transcript variant 3
UniProt
P07196; Q9UN70
Immunogen
The original human IgG1 version of this antibody was obtained in the following manner: First, white matter tissue was dissected at autopsy from subjects with clinically defined MS, and individual B lineage cells were identified and captured. Multiplex PCR was used to amplify the Ig heavy and light chain variable regions, and recombinant IgGs were prepared from the Ig variable region sequences with IgG1 heavy chain and kappa constant domains.
Application Notes
The original human IgG1 version of the recombinant antibody MS-C2 was constructed in order to explore the antigen specificity of the Multiple Sclerosis B cell response (Willis et al, 2015; pmid: 26648933). The antibody was shown to bind two important neural antigens: neurofilament light (NF-L; strong binding with absorbance values that exceeded the mean +2SD of the control data set) and protocadherin PCDHGC3 (Willis et al, 2015; pmid: 26648933). Protocadherin gamma subfamily C, three (PCDHGC3) transcript variant three was of particular interest as a candidate autoantigen as protocadherin isoforms are predominantly expressed in the nervous system and have been implicated in human neurological disorders.
Antibody First Published
Willis et al. Investigating the Antigen Specificity of Multiple Sclerosis Central Nervous System-Derived Immunoglobulins Front Immunol. 2015 Nov 25;6:600 PMID:26648933
Note on publication: Describes the application of recombinant antibodies from CNS lesion-derived B cells in antigen discovery in Multiple Sclerosis.
Note on publication
Describes the application of recombinant antibodies from CNS lesion-derived B cells in antigen discovery in Multiple Sclerosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
