Cat. No.
MABL-058
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
RG/26
From
Recombinant Antibody
Specificity
This antibody recognises human Spectrin which is a cytoskeletal protein creating scaffolding of the plasma membrane.
Alternative Names
Beta-I spectrin; Spectrin beta chain, erythrocytic; EL3; HS2; HSPTB1; membrane cytoskeletal protein; erythrocyte
UniProt
P11277
Immunogen
This antibody was raised by immunising mice with red cell membranes.
Application Notes
IHC: RG/26 antibody was used to stain membranes of iRBCs infected with a parasite in a study which investigated influence of vascular endothelial growth factor on red cells infected with Plasmodium falciparum (Sachanonta et al., 2008). WB: This antibody was also used in Western blot analysis to assess free and degraded spectrin as an element of a study which aimed at investigating the infuence of constant storage temperatures and multiplewarming cycles on stored red blood cells (Wagner et al., 2014).
Antibody First Published
Sachanonta et al. Host vascular endothelial growth factor is trophic for Plasmodium falciparum-infected red blood cells. Asian Pac J Allergy Immunol. 2008 Mar;26(1):37-45.PMID:18595528
Note on publication
This article describes the earliest identified use of RG/26 antibody to stain plasma membranes of iRBCs in a study which investigated influence of vascular endothelial growth factor on red cells infected with Plasmodium falciparum.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
