Cat. No.
MABL-059
Application
crystallography, FSEC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1F4
From
Recombinant Antibody
Specificity
This antibody recognises α1β2γ2 GABA-A receptor which is found in up to 50% of the synapses in the brain. It's ligand, GABA is a major inhibitory transmitter in the nervous system, binds to this receptor and opens a channel permeable to chloride. Disfunctions of GABAR are found in anxiety, neurodevelopmental disorderds and epilepsy.
Alternative Names
Gamma-aminobutyric acid receptor A; Gamma-aminobutyric acid receptor; Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit gamma-2; GABA(A); GABA-A receptor; α1β2γ2 GABA-A receptor
UniProt
P14867; P47870; P18507
Immunogen
This antibody was raised against α1β2γ2 GABA-A receptor.
Application Notes
FSEC: High affinity and specificity of the 1F4 antibody to α1β2γ2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the α1β2γ2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the α1β2γ2 GABA-A receptor (Zhu et al., 2018).
Antibody First Published
Zhu et al. Structure of a human synaptic GABA-A receptor Nature. 2018 Jul;559(7712):67-72. doi: 10.1038/s41586-018-0255-3. Epub 2018 Jun 27. PMID:29950725
Note on publication
This article describes the generation of 1F4 antibody as well as Fab fragments derived from it for the pupose of puryfying and analysing the structure of α1β2γ2 GABA-A receptor.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
