Cat. No.
MABL-060
Application
RIA
Isotype
Engineer antibody
Species Reactivity
n/a
Clone No.
Clone D
From
Recombinant Antibody
Specificity
Thi antibody recognises haloperidol which is one of the major antipsychotic drugs. It is a D2 dopamine receptor antagonist and is widely used in treatment of schizophrenia, psychoses and mania. This antibody binds to the p-chlorophenyl ring (ring 2) of haloperidol and prefers an extended conformer of the drug molecule.
Alternative Names
Haldol; 258(2)-1; D; Einalon S; Eukystol; Aloperidin; Bioperidolo; Brotopon; Dozic; Duraperidol; Halosten; Keselan; Linton; Peluces; Serenace; Sigaperidol
UniProt
Immunogen
This antibody was raised by immunising mice with a haloperidol coupled to BSA via an aminooxyacetic acid arm through the keto group of the butyrophenone chain (oxime conjugate).
Application Notes
RIA: radioligand binding assays were performed to asses binding affinity of the anti- haloperidol D antibody (Kd=4.4 nM). This antibody possesses a binding site which is highly sensitive to the electron-withdrawing characteristics of the substituents on ring 2 (Sherman et al., 1986).
Antibody First Published
Bolger et al. Preparation and characterization of antisera and monoclonal antibodies to haloperidol. Immunol Invest. 1985 Dec;14(6):523-40. PMID:2420719
Note on publication
This article describes the preparation of the monoclonal antibodies which specifically bind haloperidol, among them this 258(2)-i antibody (later renamed as anti-haloperidol antibody D).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
