Cat. No.
MABL-062
Application
antibody imprint, EM ICC, ELISA
Isotype
Engineer antibody
Species Reactivity
Bovine, Human
Clone No.
K42-41L
From
Recombinant Antibody
Specificity
This antibody recognises secifically C3 loop of metarhodopsin-I (residues 230-252) (and also dark-adapted form of rhodopsin) but it does not bind to the ligh-activated form - metarhodopsin-II. Rhodopsin is the best studied GPCR and is present in rod cells where it is activated by light being responsible for the signal transduction in the visual system.
Alternative Names
opsin-2; meta I rhodopsin; rhodopsin; opsin 2; metarhodopsin I
UniProt
P08100
Immunogen
This antibody was raised by immunising mice with rhodopsin.
Application Notes
Antibody imprint: This antibody was used to investigate the structure of rhodopsin's meta-states; the researchers employed the antibody imprint technique to analyse conformational changes which this molecule undergoes upon activation by light (Bailey et al., 2003). Another group used K42-41L antibody to demonstrate that it is capable of discriminating between metarhodopsins I and II, and that it stabilises metarhodopsin I while inhibiting metarhodopsin II; these data suggest that this antibody might be useful in the structural studies of opsins (Piscitelli et al., 2006). EM ICC: K42-41L antibody was utilised to stain different structures of Xenopus laevis retinas in EM immunocytochemistry LR white samples in a study investigating opsin localisation (Besharse and Wetzel, 1995).
Antibody First Published
Adamus et al. Use of peptides to select for anti-rhodopsin antibodies with desired amino acid sequence specificities. Pept Res. 1988 Sep-Oct;1(1):42-7. PMID:2485225
Note on publication
This article describes the generation and characterisation of the K42-41L antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
