Cat. No.
MABL-075
Application
IB, IP, IHC
Isotype
Engineer antibody
Species Reactivity
C. elegans, Rat, Mouse
Clone No.
K57/1
From
Recombinant Antibody
Specificity
This antibody is specific for the ectodomain of the Kv4.2 potassium channel. It does not react with Kv4.3 and Kv4.1.
Alternative Names
K57/1R; Potassium voltage-gated channel subfamily D member 2; Voltage-gated potassium channel subunit Kv4.2; Kcnd2; Kiaa1044; MNCb-7013; RK5; Shal1
UniProt
Q9NZV8
Immunogen
This antibody was raised by immunising BALB/c mice with a synthetic peptide corresponding to the extracellular S1-S2 loop (amino acids 209-225) of human Kv4.2, where the third Ser was substituted in place of a Cys for peptide conjugation purposes.
Application Notes
This antibody has been used in immunoblot analysis of crude membranes from rat and mouse brain (Andrews et al, 2019; PMID:30667360), as well as crude C. elegans extracts (Andrews et al, 2019; PMID:30667360) and lysates of COS-1 cells transfected to express Kv4.2 (Shibata et al, 2003). It has also been used in immunohistochemical analysis of adult rat and mouse brain sections (Sack et al, 2013; Andrews et al, 2019; PMID:30667360), and immunofluorescence analysis of surface Kv4.2 expression by Kv4.2-transfected COS-1 cells (Shibata et al, 2003). Using ELISA, pre-incubation with soluble Kv4.2 peptide has been shown to block the binding of this antibody to its antigen (Sack et al, 2013). This antibody, when labelled with gold, has been used in electron microscopy to assess endogenous Kv4.2 expression in rat hippocampal sections (Kim et al, 2007).
Antibody First Published
Shibata et al. A Fundamental Role for KChIPs in Determining the Molecular Properties and Trafficking of Kv4.2 Potassium Channels. J Biol Chem. 2003;278(38):36445‐36454.PMID:12829703
Note on publication
Describes the original generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
