Cat. No.
MABL-076
Application
IB, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Vertebrates
Clone No.
K58/35
From
Recombinant Antibody
Specificity
This pan-voltage-gated sodium channel antibody is specific for a conserved sequence present in all vertebrate Nav1 isoforms. It binds to all voltage-gated sodium channel isoforms.
Alternative Names
K58/35R; sodium voltage-gated channel; Na+ channel
UniProt
Immunogen
This antibody was raised by immunising mice with a synthetic peptide corresponding to a highly conserved segment of the intracellular III–IV loop of vertebrate Na+ channels.
Application Notes
This antibody has been used to stain teased axons prepared from rat spinal nerves (Devaux, 2012), sections of rat retina (Wart et al, 2005) and mice optic nerve cryosections (Vega et al, 2008) for Nav expression. This antibody has been shown to label Nav channels concentrated on the axon initial segment and nodes of Ranvier (Andrews et al, 2019). This antibody has been used in immunoblot analysis of rat brain membranes (Wart et al, 2005; Vega et al, 2013), and to stain COS-1 cells transfected with Nav1 cDNA (Wart et al, 2005). Additionally, this antibody has been used in western blot analysis of mice optic nerve and brain membrane proteins (Vega et al, 2008).
Antibody First Published
Rasband et al. Dependence of Nodal Sodium Channel Clustering on Paranodal Axoglial Contact in the Developing CNS Journal of Neuroscience 1 September 1999, 19 (17) 7516-7528; PMID:10460258
Note on publication
Describes the original generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
