Cat. No.
MABL-096
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
N135/37.2
From
Recombinant Antibody
Specificity
This antibody is specific for a sequence of amino acids between the 226th and 421th amino acid of the JIP-2 gene. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAP
Alternative Names
N135/37.2R; N135/37; C-Jun-amino-terminal kinase-interacting protein 2; JIP-2; JNK-interacting protein 2; Islet-brain-2; IB-2; JNK MAP kinase scaffold protein 2; Mitogen-activated protein kinase 8-interacting protein 2
UniProt
Q9ERE9
Immunogen
This antibody was raised by immunising BALB/c mice with Fusion protein corresponding to Mouse IB-2 aa 226-421.
Application Notes
This antibody is recommended for the detection and analysis of JIP-2/IB-2 by western blot and immunohistochemistry. For instance, the mouse version of this antibody was used to detect JIP- 2/IB-2 by western blot in adult rat brain membranes. And it was also used to detect JIP-2/IB-2 by immunohistochemistry in adult rat brain membranes.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
