Cat. No.
MABL-099
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
N150/21.3
From
Recombinant Antibody
Specificity
This antibody is specific for the Doc2b protein and it does not show any cross reactivity with the Doc2a protein. Doc2b is a calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes.
Alternative Names
N150/21.3R; N150/21; Double C2-like domain-containing protein beta; Doc2-beta
UniProt
P70610
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein corresponding to amino acids 1-216 (N-terminus) of rat Doc2b.
Application Notes
This antibody is recommended for the detection and analysis of Doc2b by western blot and immunohistochemistry. For instance, the mouse version of this antibody was used to detect Doc2b by western blot in extracts of RBM and COS cells transiently transfected with GFP-tagged Doc2b or untagged Kv2.1 plasmids. Besides western blot it was also used to detect Doc2b in adult rat hippocampus using immunohistochemistry.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
