Cat. No.
MABL-101
Application
ICC, WB, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
N153/5.1
From
Recombinant Antibody
Specificity
This antibody is specific for the mitofusion-2 protein and shows no cross reactivity with mitofusion-1. Mitofusion-2 is a mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion
Alternative Names
N153/5.1R; N153/5; Hypertension-related protein 1; Mitochondrial assembly regulatory factor; HSG protein; Transmembrane GTPase MFN2
UniProt
Q80U63
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein corresponding to Mouse Mitofusin 2 aa 370-600.
Application Notes
This antibody is recommended for the detection and analysis of mitofusin-2 by western blot, immunofluorescence and immunocytochemistry. For instance, the mouse version of this antibody was used to detect Mitofusin-2 by western blot in extract of crude cardiac mitochondrial preps from WT, Mfn1 KO or Mfn2 KO mice. It was also used for immunohistochemistry in which Mitofusin-2 was detected in adult rat hippocampus.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
