Cat. No.
MABL-103
Application
ICC, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
N168/6.3
From
Recombinant Antibody
Specificity
This antibody is specific for the Navbeta4 protein in mice and rats. This protein Modulates channel gating kinetics.
Alternative Names
N168/6.3R; N168/6; Sodium channel subunit beta-4; Scn4b; Navbeta4
UniProt
Q7M730
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 184- 228 (cytoplasmic C-terminus) of rat Navbeta4.
Application Notes
This antibody is recommended for the detection and analysis of Navbeta4 Na+ channel by immunocytochemistry, western blot and immunohistochemistry. For instance, the mouse version of this antibody was used to detect Navbeta4 Na+ channel by western blot in extracts of RBM and COS cells transiently transfected with GFP-tagged Navbeta4 or untagged Kv2.1 plasmid. Furthermore it was used to detect Navbeta4 Na+ channel by immunocytochemistry in adult rat sciatic nerves. And finally it was also used for detection of Navbeta4 Na+ channel by immunohistochemistry in adult rat brain.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
