Cat. No.
MABL-126
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
N28/9
From
Recombinant Antibody
Specificity
This antibody is specific for the Vglut1 protein. The antibody does not cross-react with VGlut2 or VGlut3. Vglut1 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Alternative Names
N28/9R; Vesicular glutamate transporter 1; Slc17a7; Brain-specific Na(+)- dependent inorganic phosphate cotransporter; Solute carrier family 17 member 7
UniProt
Q62634
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 493- 560 (cytoplasmic C-terminus) of rat VGlut1
Application Notes
Immunofluorescense was preformed on the brain of mice and rats using this antibody (Lindhoff et al, 2009; PMID:19285470) (Garcia-Pino et al, 2009; PMID:20151361) (Takahashi et al, 2011; PMID:21262467). This antibody has been used for immunohistochemistry on the brain of a rat (Larsson et al, 2011; PMID:21810784). The antibody was used in a western blot experiment on rat brain extracts (Garcia-Pino et al, 2009; PMID:20151361). Immunocytochemistry has been preformed on normal rat retinas with this antibody (Caminos et al, 2015; PMID:25499209).
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
