Cat. No.
MABL-128
Application
ICC, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Chicken, Rat, Torpedo californica, Human, Mouse
Clone No.
N29/29
From
Recombinant Antibody
Specificity
This antibody is specific for the Vglut1 protein. The antibody does not cross-react with VGlut1 or VGlut3. Vglut2 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells.
Alternative Names
N29/29R; Vesicular glutamate transporter 2; Slc17a6; Differentiation- associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6
UniProt
Q9JI12
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 501- 582 (cytoplasmic C-terminus) of rat VGlut2.
Application Notes
The IgG1 antibody was used for immunofluorescense on the brain of a chicken (González-Cabrera et al, 2015; PMID:25594665). Immunohistochemistry was preformed on the brain of a chicken using the IgG1 version of this antibody (González-Cabrera et al, 2015; PMID:25594665). The IgG1 variant of this antibody was used in a immunocytochemistry experiment on human neurons (Fantuzzo et al, 2017; PMID:28781993). Immunofluorescense was preformed with the IgG1 version of this antibody on the neurons of a rat (Dabrowska et al, 2013; PMID:24009552). The IgG1 version of this antibody was used to preform western blot and immunofluorescense on the electroplaque of Torpedo californica (Li and Harlow, 2014; PMID:24744885)
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
