Cat. No.
MABL-129
Application
ICC, WB
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
N291C/22
From
Recombinant Antibody
Specificity
This antibody is specific for exon 1a/5 junction of TRIP8b. TRIP8b is an accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, regulating their cell-surface expression and cyclic nucleotide dependence.
Alternative Names
N291C/22R; PEX5-related protein; PEX5-like protein; Peroxin-5-related protein; TPR-containing Rab8b-interacting protein; Tetratricopeptide repeat-containing Rab8b-interacting protein; Pex5Rp
UniProt
Q925N3
Immunogen
This antibody was raised by immunising BALB/c mice with a synthetic peptide amino acids 1- 12 (MYQGHMQLVNEQ, exon 1a (yellow) and exon 5 (green) of constant region) of mouse TRIP8b.
Application Notes
This antibody is recommended for the detection and analysis of TRIP8b by immunocytochemsitry and western blot. For instance, the mouse version of this antibody was used to detect TRIP8b by western blot in extracts of COS cells transiently transfected with carboxyterminally (C) and amino-terminally (N) GFPtagged TRIP8b-1a-5, TRIP8b-1a-4 or untagged Kv2.1 plasmid. Furthermore TRIP8b was also detected by western blot in crude membranes from adult rat brain and WT and TRIP8b KO mouse brains probed with N291C/22 and N52A/42 TC supe.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
