Cat. No.
MABL-149
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Bovine, Deer, Sheep, Syrian Hamster, Human, Mouse
Clone No.
P-clone
From
Recombinant Antibody
Specificity
This antibody binds amino acid residues 90-120 [ amino acids HG(S,N)QWNKPSKPKTN] or epitope I of the bovine prion protein. This epitope is also present in human PrP as well as in all ungulate PrP sequences, including cattle, deer, elk, kudu, goat, and sheep.This antibody doesnt bind the native disease- associated PrP conformer. It can only recognise the denatured or pretreated PrP, prepared by denaturation and/or proteolysis for peptide-like presentation.
Alternative Names
CD230; PRNP; Major prion protein; Major scrapie-associated fibril protein 1
UniProt
P10279
Immunogen
The original antibody was generated by immunizing mice with amino acid sequence 96-105 of bovine prion protein.
Application Notes
Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative prion disease affecting cattle that is transmissible to humans, manifesting as a variant of Creutzfeldt-Jakob disease (vCJD) likely following the consumption of meat contaminated with BSE prions. This high affinity antibody can be used for detection of infectious prion in a sample. P clone has been used in the development of conformation dependent immunoassay for detection of prion protein in a sample. It can recognise prions from cow, sheep, human, deer, hamster in a western blot assay (PMID:12389035). P clone binds peptide derived from the unstructured region (aa90-105) of bovine PrP (BoPrP) with an affinity of 1pM (PMID: 16962610).
Antibody First Published
Safar et al. Measuring prions causing bovine spongiform encephalopathy or chronic wasting disease by immunoassays and transgenic mice. Nat Biotechnol (2002); 20(11):1147-50.PMID:12389035
Note on publication
Describes the generation of conformation specific antibodies towards bovien prion
protein to be used in a conformation dependant immunoassay.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
