Cat. No.
MABL-148
Application
IP, WB, IF
Isotype
Engineer antibody
Species Reactivity
Rat, worm, Human, Mouse
Clone No.
N52A/42.1
From
Recombinant Antibody
Specificity
This antibody is specific for the mortalin protein. Mortalin is a chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis.
Alternative Names
N52A/42.1R; Stress-70 protein, mitochondrial; Hspa9; 75 kDa glucose- regulated protein; GRP-75; Heat shock 70 kDa protein 9; Mortalin; Peptide-binding protein 74; PBP74; p66 MOT
UniProt
P38647
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 551- 766 of mouse SALM2. The antibody was off traget.
Application Notes
The IgG1 version of this antibody was used in a western blot on COS-7 cells and on COS-1 cells (Vincente et al, 2016; PMID:28322444) (Thiffault et al, 2015; PMID:26503721).Immunofluorescense experiments with the IgG1 version of this antibody as positive control were preformed on the mouse brain (Kirmiz et al, 2018; PMID:30012696). The IgG1 version of this antibody has been used for immunoprecipitation on rat brain extracts (Bishop et al, 2018; PMID:29403353).
Antibody First Published
Gong et al Developing high-quality mouse monoclonal antibodies for neuroscience research - approaches, perspectives and opportunities N Biotechnol. 2016 Sep 25;33(5 Pt A):551-64. PMID:26644354
Note on publication
Describes the need for good antibodies usable is neurological research and the creation of these antibodies
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
