Cat. No.
MABL-147
Application
ICC, IP, WB
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
N51/6.2
From
Recombinant Antibody
Specificity
This antibody is specific for NGL-3/LRRC4B. The antibody shows no cross-reactivity against NGL-1 or NGL-2. NGL-3/LRRC4B is a synaptic adhesion protein. It regulates the formation of excitatory synapses.
Alternative Names
N51/6.2R; Leucine-rich repeat-containing protein 4B; N51/6; Lrrc4b; Netrin-G3 ligand; NGL-3
UniProt
P0CC10
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 598- 709 of rat NGL-3.
Application Notes
This antibody is recommended for detection and analysis of NGL-3 by immunocytochemistry, immunopercipitation and western blot. For instance, the mouse version of this antibody was used to detect NGL-3 by western blot in extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2 NGL-3 or untagged Kv2.1 plasmid.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
