Cat. No.
MABL-146
Application
ICC; IHC; WB
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N419/78
From
Recombinant Antibody
Specificity
This antibody binds to most Nav1 channels. Nav1 channels mediate the voltage-dependent sodium ion permeability of excitable membranes.
Alternative Names
N419/78R; Sodium channel protein type 1 subunit alpha; Sodium channel protein brain I subunit alpha; Sodium channel protein type I subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.1; SCN1A
UniProt
P35498
Immunogen
This antibody was raised by immunising BALB/c mice with a synthetic peptide amino acids 1501-1518 (TEEQKKYYNAMKKLGSKK, cytoplasmic loop between repeat III and IV) of human Nav1.1.
Application Notes
This antibody is recommended for detection and analysis of Nav1 Na+ channel by immunocytochemistry, immunohistochemistry and western blot. For instance, the mouse version of this antibody was used to detect nav1 Na+ channel by western blot in fractions of adult rat brain membranes and extracts of COS or HEK cells transiently or stably expressing different untagged Nav1.X or Kv2.1 plasmid.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
