Cat. No.
MABL-151
Application
ICC, IP, KO, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N59/36.1
From
Recombinant Antibody
Specificity
This antibody is specific for the NR2B protein, a component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage- dependent sensitivity to magnesium.
Alternative Names
N59/36.1R; GluN2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B
UniProt
Q00960
Immunogen
This antibody was raised by immunising BALB/c mice with a fusion of protein amino acids 20- 271 (extracellular N-terminus) of rat NR2B.
Application Notes
The IgG2a version of this antibody has been used in western blotting on rat cortical neurons (Plowey et al, 2014; PMID: 24874075). Co-immunoprecipitation has been preformed on extrasynaptic membranes of adult rat brains with the IgG2A version of this antibody (Chiu et al, 2019; PMID:31291571). This antibody has shown use in immunocytochemistry, immunofluorescence, immunohistochemistry or for knock out.
Antibody First Published
Plowey et al. Mutant LRRK2 Enhances Glutamatergic Synapse Activity and Evokes Excitotoxic Dendrite Degeneration Biochim Biophys Acta. 2014 Sep; 1842(9): 1596–1603.PMID:24874075
Note on publication
Describes research on the mutations of the LRRK2 gene. These mutations might have something to do with parkinsons disease.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
