Cat. No.
MABL-152
Application
IP, WB, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N7/18.1
From
Recombinant Antibody
Specificity
This antibody is specific for the Cav1 protein, regulatory subunit of L-type calcium channels. Regulates the activity of L-type calcium channels that contain CACNA1A as pore-forming subunit (By similarity).
Alternative Names
N7/18.1R; Voltage-dependent L-type calcium channel subunit beta-1; CAB1; Calcium channel voltage-dependent subunit beta 1
UniProt
P54283
Immunogen
This antibody was raised by immunising BALB/c mice with a synthetic peptide of amino acids 19-34 of rat Cav1.
Application Notes
The IgG2a version of this antibody was used for immunofluorescence on primary myoblasts from 1-null muscles (5, 9) infected with 1a cDNA-containing virions at a multiplicity of infection of 0.5 (Eltit et al, 2014; PMID:25384984). Immunoprecipitation has been preformed on HEK293 cell lysates using the IgG2a version of this antibody (Morgenstern et al, 2019; PMID:31403402). This antibody has been used for immunofluorescence on mouse hippocampal cultures (Held et al, 2020; PMID:32616470).
Immunoprecipitation was preformed with this antibody on the lysate of mouse hemibrains ( Voskobiynyk, 2020; PMID:32657270). This antibody was used in immunoblotting of myoblasts stably transduced with Wt- β1a or β1a-V156A subunit (Perez, 2018; PMID:29212769). Immunofluorescence was preformed on mouse diaphraghms by using this antibody (Kaplan et al, 2018; PMID:29949772).
Antibody First Published
Eltit et al. Amino acid residues 489-503 of dihydropyridine receptor (DHPR) β1a subunit are critical for structural communication between the skeletal muscle DHPR complex and type 1 ryanodine receptor J Biol Chem. 2014 Dec 26;289(52):36116-24. PMID:25384984
Note on publication
investigating the role of the C-terminal end of The β1a subunit in the functional and structural communication between the DHPR and the Ca(2+) release channel (RyR1).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
