Cat. No.
MABL-182
Application
WB, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
L18A/3R
From
Recombinant Antibody
Specificity
This antibody is specific for BKBeta4. It shows no cross-reactivity against BKBeta1, BKBeta2 or BKBeta3. BKBeta4 is a regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. It modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. It decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation kinetics. May decrease KCNMA1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. Makes KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations.
Alternative Names
Calcium-activated potassium channel subunit beta-4; Kcnmb4; BK channel subunit beta-4; BKbeta4; Calcium-activated potassium channel, subfamily M subunit beta-4; Charybdotoxin receptor subunit beta-4; K(VCA)beta-4; Maxi K channel subunit beta-4; Slo-beta-4
UniProt
Q9JIN6
Immunogen
This antibody was raised by immunizing a mouse with fusion protein amino acids 45-167 (entire extracellular domain) of mouse BKBeta4.
Application Notes
This antibody was used for immunoblotting on brain tissue from rats exposed to CVS and mouse hippocampi (Yu et al, 2018; PMID:30271963). Immunofluorescence was preformed on HEK293 cells and N2a neurons with this antibody (Chen et al, 2013; PMID:23504458). Transfected HEK293 cells were immunoblotted with this antibody (Chen et al, 2013; PMID:23504458). The hippocampi of rats were immunostained with this antibody. This assay was preformed in order to assess the integrity of mossy fiber pathway and whether MaxiK channel are expressed at mossy fiber axon and terminals (Cox et al, 2014; PMID:24486049). DRG (L4/L5) from rats was used for a western blot with this antibody (Zhang et al, 2012; PMID:22607137).
Antibody First Published
Zhang et al. Inflammation-induced changes in BK(Ca) currents in cutaneous dorsal root ganglion neurons from the adult rat Mol Pain. 2012 Jul 4;8:37. PMID:22607137
Note on publication
This study provides additional support for the conclusion that it may be possible, if not necessary, to selectively treat pain arising from specific body regions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
