Cat. No.
MABL-183
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
L51/82R
From
Recombinant Antibody
Specificity
This antibody is specific for the MMP9 protein. MMP9 could play a role in bone osteoclastic resorption. It cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments.
Alternative Names
Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; L51/82
UniProt
P50282
Immunogen
The original version of this antibody was raised by immunizing a mouse with human MMP-9 residues 107– 445. Then the hybridomas were sequenced by addgene and based on the variable domain sequences new recombinant formats were created.
Application Notes
Small bowel and colonic muscularis lysates from C57Bl/6 mice were immunoblotted with this antibody (Moore et al, 2011; PMID:21703213). This antibody was used for immunofluorescence on postnatal brains of 125 Sprague-Dawley rats (Aujla and Huntley, 2014; PMID:24114974). Immunoblot was preformed with this antibody on adult rat whole-hippocampal lysates (Aujla and Huntley, 2014; PMID:24114974). An immunoblot was preformed on HEK293 cells with this antibody (Duellman et al, 2012; PMID:22942228). This antibody was used for immunohistochemistry on HEK293 cells (Duellman et al, 2014; PMID:24627221). A western blot was preformed with this antibody on HEK293 cells (Duellman et al, 2014; PMID:24627221). Mice brain sections were used for immunohistochemistry with this antibody (Kuo et al, 2020; PMID:32926126).
Antibody First Published
Moore et al. Matrix metalloproteinase-9 inhibition reduces inflammation and improves motility in murine models of postoperative ileus Gastroenterology. 2011 Oct;141(4):1283-92, 1292.e1-4. PMID:21703213
Note on publication
Mice and rats underwent laparotomy and bowel manipulation; bowel tissues were collected 3 to 24 hours later and analyzed by real-time reverse-transcriptase polymerase chain reaction, immunoblot, in situ zymography, and functional analyses.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
