Cat. No.
MABL-184
Application
WB
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N415/24R
From
Recombinant Antibody
Specificity
This antibody is specific for GABA-A-R-Alpha5. It does not cross-react with GABA-A-R-Alpha2. GABA-A-R-Alpha5 is a ligand-gated chloride channel subunit which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. May be involved in GABA-A receptor assembly, and GABA-A receptor immobilization and accumulation by gephyrin at the synapsee.
Alternative Names
Gamma-aminobutyric acid receptor subunit alpha-5; GABRA5; GABA(A) receptor subunit alpha-5; N415/24
UniProt
P31644
Immunogen
This antibody was raised by immunizing a mouse with fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA-A-R-Alpha5 receptor subunit alpha-5 and Gabra5.
Application Notes
This antibody was used for a western blot on mouse cell lysates (Davenport et al, 2020; PMID:33096025). Western blot was preformed on mouse hippocampal protein samples with this antibody (Gruol et al, 2018; PMID:29787738). Lysates from L3 to L5 DRG and the ipsilateral dorsal section of rats were used for western blot with this antibody (Franco-Enzástiga et al, 2020; PMID:32750173).
Western blot was preformed on human brain, and on KO mouse brain with this antibody (Gong et al, 2016; PMID:26644354).
Antibody First Published
Gong et al. Developing high-quality mouse monoclonal antibodies for neuroscience research - approaches, perspectives and opportunities N Biotechnol. 2016 Sep 25;33(5 Pt A):551-64. PMID:26644354
Note on publication
A methodology of multi-step mAb screening focused on identifying mAbs exhibiting efficacy and specificity in labeling mammalian brain samples is described.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
