Cat. No.
MABL-271
Application
IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
H184
From
Recombinant Antibody
Specificity
This antibody binds mouse peripherin, but also cross reacts with human and rat peripherin.
Peripherin is a type III intermediate filament protein expressed mainly in neurons of the peripheral nervous system.
Alternative Names
PRPH; PRPH1
UniProt
P15331
Immunogen
The original hybridoma was generated by fusing islet-infiltrating B lymphocytes collected from F1(NODxNOR) mouse developing insulitis and the NS-1 myeloma cell line in polyethylene glycol (PEG).
Application Notes
This antibody was used for the immunofluorescence staining of peripherin from cryosections (5 um) of pancreata from NOD.RAG-2/ mice, Sprague Dawley rats, and human cadaveric organ donors. The reactivity of this antibody towards several NOD.RAG-2/ mouse organs was also determined using immunofluorescence staining (PMID: 15616012).
Antibody First Published
Carrillo et al. Anti-peripherin B lymphocytes are positively selected during diabetogenesis. Mol Immunol. 2008 Jun;45(11):3152-62. PMID:18433871
Note on publication
The original publication describes the generation of hybridoma cell lines of islet- infiltrating B-cells from mice with type 1 diabetes and diabetes-resistant mice, and the characterization of the tissue specificity of the antibodies produced by these hybridomas.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
