Anti-Spectrin mAbs

Cat. No.

MABL-058

Name

Anti-Spectrin mAbs

Clone No.

AFD-RG/26

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody recognises human Spectrin   which is a cytoskeletal protein creating scaffolding of the plasma membrane.

Alternative Name

Beta-I spectrin; Spectrin beta chain,   erythrocytic; EL3; HS2; HSPTB1; membrane cytoskeletal protein; erythrocyte

UniProt

P11277

Immunogen

This antibody was raised by immunising mice   with red cell membranes.

Application Notes

IHC: RG/26 antibody was used to stain   membranes of iRBCs infected with a parasite in a study which investigated   influence of vascular endothelial growth factor on red cells infected with   Plasmodium falciparum (Sachanonta et al., 2008). WB: This antibody was also   used in Western blot analysis to assess free and degraded spectrin as an   element of a study which aimed at investigating the infuence of constant   storage temperatures and multiplewarming cycles on stored red blood cells   (Wagner et al., 2014).

Antibody First Published

Sachanonta et al. Host vascular   endothelial growth factor is trophic for Plasmodium falciparum-infected red   blood cells. Asian Pac J Allergy Immunol. 2008 Mar;26(1):37-45.PMID:18595528

Note on publication

This article describes the earliest   identified use of RG/26 antibody to stain plasma membranes of iRBCs in a   study which investigated influence of vascular endothelial growth factor on   red cells infected with Plasmodium falciparum.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.