Key features and details | |
Cat. No. | MABL-059 |
Name | Anti-GABAR mAbs |
Clone No. | AFD-1F4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, FSEC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognises α1β2γ2 GABA-A receptor which is found in up to 50% of the synapses in the brain. It's ligand, GABA is a major inhibitory transmitter in the nervous system, binds to this receptor and opens a channel permeable to chloride. Disfunctions of GABAR are found in anxiety, neurodevelopmental disorderds and epilepsy. |
Alternative Name | Gamma-aminobutyric acid receptor A; Gamma-aminobutyric acid receptor; Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit gamma-2; GABA(A); GABA-A receptor; α1β2γ2 GABA-A receptor |
UniProt | P14867; P47870; P18507 |
Immunogen | This antibody was raised against α1β2γ2 GABA-A receptor. |
Application Notes | FSEC: High affinity and specificity of the 1F4 antibody to α1β2γ2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the α1β2γ2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the α1β2γ2 GABA-A receptor (Zhu et al., 2018). |
Antibody First Published | Zhu et al. Structure of a human synaptic GABA-A receptor Nature. 2018 Jul;559(7712):67-72. doi: 10.1038/s41586-018-0255-3. Epub 2018 Jun 27. PMID:29950725 |
Note on publication | This article describes the generation of 1F4 antibody as well as Fab fragments derived from it for the pupose of puryfying and analysing the structure of α1β2γ2 GABA-A receptor. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
