Anti-DNMT3L mAbs

Cat. No.

MABL-093

Name

Anti-DNMT3L  mAbs

Clone No.

AFD-N117/9.5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific of the   DNMT3L antigen. This protein is a catalytically inactive regulatory factor of   DNA methyltransferases that can either promote or inhibit DNA methylation   depending on the context (By similarity).

Alternative Name

 N117/9.5R; DNA (cytosine-5)-methyltransferase 3-like

UniProt

 Q9UJW3

Immunogen

This antibody was raised by   immunising BALB/c mice with the DNMT3L antigen.

Application Notes

This antibody is recommended for the   detection and analysis of DNMT3L by western blot, immunoflurescence and   immunohistochemistry. For instance, the mouse version of this antibody was   used to detect DNMT3L by western blot in COS cells transiently transfected   with untagged Kv2.1 and Flag- tagged DNMT3L. It was also used to detect   DNMT3L by immunofluorescence in COS cells expressing Myc- tagged human   DNMT3L.

Antibody First Published

Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

This article describes the   generation of a library of recombinant monoclonal antibodies (R-mAbs) from a   pool of mAb-producing hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.