Key features and details | |
Cat. No. | MABL-157 |
Name | Anti-Laforin mAbs |
Clone No. | AFD-N84/37.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, KO, WB, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the human laforin protein. Laforin plays an important role in preventing glycogen hyperphosphorylation and the formation of insoluble aggregates, via its activity as glycogen phosphatase, and by promoting the ubiquitination of proteins involved in glycogen metabolism via its interaction with the E3 ubiquitin ligase NHLRC1/malin. |
Alternative Name | N84/37.1R; EPM2A; Glucan phosphatase; Glycogen phosphatase; Lafora PTPase; LAFPTPase |
UniProt | O95278 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 1-331 (full-length) of human Laforin. |
Application Notes | This antibody is recommended for detection and analysis of laforin by immunoblotting, immunocytochemistry, immunohistochemistry and knockout. For instance, the mouse version of this antibody was used to detect laforin by western blot in crude membrane fractions from whole rat or mouse brain and from human hippocampus, cerebral cortex or cerebellum. |
Antibody First Published | Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360 |
Note on publication | Describes the generation of a library of recombinant monoclonal antibodies (R- mAbs) from a pool of mAb-producing hybridomas for neuroscience research. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
