Anti-GFP mAbs

Cat. No.

MABL-159

Name

Anti-GFP mAbs

Clone No.

AFD-N86/38.1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, IF, IHC

Species Reactivity

Aequorea victoria

Basic Information

Specificity

This antibody is specific for amino acids 1-238 (full-length) of jellyfish green fluorescent protein. It cross-reacts   with cyan and yellow fluorescent protein. It does not cross-react with red   fluorescent protein. Energy-transfer acceptor. Its role is to transduce the   blue chemiluminescence of the protein aequorin into green fluorescent light   by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

Alternative Name

N86/38.1R; Green fluorescent protein

UniProt

P42212

Immunogen

This antibody was raised by immunising   BALB/c mice with a fusion of protein amino acids 1- 238 (full-length) of   jellyfish green fluorescent protein.

Application Notes

Immunofluorescence was preformed with this   antibody in the brain of mice. Here it was bound to both yellow and green   fluorescent protein (Kumar et al, 2015; PMID:25337655). This antibody was   used for immunoprecipitation on Elav-GAL4, 50Y-GAL4, UAS-eGFP, The UAS-GFP and RpL10A fly strains of the genus Drosophila (Thomas et al, 2012;   PMID:22792260). Western blotting was preformed with this antibody on samples   of Elav-GAL4, 50Y-GAL4, UAS-eGFP, The UAS-GFP and RpL10A fly strains of the   genus Drosophila (Thomas et al, 2012; PMID:22792260). Immunohistochemistry was preformed on zebrafish to visualize GFP (Mackenzie and Raible, 2012;   PMID:23094043). Immunoprecipitation using this antibody was preformed on the   sciatic nerves of rats infected with an adenovirus containing a GFP construct   (Chang et al, 2014; PMID:25362471).

Antibody First Published

Thomas et al. A versatile method for   cell-specific profiling of translated mRNAs in Drosophila PLoS One.   2012;7(7):e40276. PMID:22792260

Note on publication

Transgenic strains of Drosophila   melanogaster expressing a GFP-tagged ribosomal protein, RpL10A, under the   control of the UAS promoter to perform cell-type specific translatome   profiling were created. The GFP::RpL10A fusion protein incorporates efficiently   into ribosomes and polysomes.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.