Anti-BK beta4 K+ channel mAbs

Cat. No.

MABL-182

Name

Anti-BK beta4 K+ channel  mAbs

Clone No.

AFD-L18A/3R

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IF

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for BKBeta4. It shows no cross-reactivity against BKBeta1, BKBeta2 or BKBeta3. BKBeta4 is a  regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. It modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby   contributing to KCNMA1 channel diversity. It decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation   kinetics. May decrease KCNMA1 channel openings at low calcium concentrations   but increases channel openings at high calcium concentrations. Makes KCNMA1   channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations.

Alternative Name

Calcium-activated potassium channel subunit   beta-4; Kcnmb4; BK channel subunit beta-4; BKbeta4; Calcium-activated   potassium channel, subfamily M subunit beta-4; Charybdotoxin receptor subunit   beta-4; K(VCA)beta-4; Maxi K channel subunit beta-4; Slo-beta-4

UniProt

Q9JIN6

Immunogen

This antibody was raised by   immunizing a mouse with fusion protein amino acids 45-167 (entire   extracellular domain) of mouse BKBeta4.

Application Notes

This antibody was used for   immunoblotting on brain tissue from rats exposed to CVS and mouse hippocampi   (Yu et al, 2018; PMID:30271963). Immunofluorescence was preformed on HEK293   cells and N2a neurons with this antibody (Chen et al, 2013; PMID:23504458).   Transfected HEK293 cells were immunoblotted with this antibody (Chen et al,   2013; PMID:23504458). The hippocampi of rats were immunostained with this   antibody. This assay was preformed in order to assess the integrity of mossy   fiber pathway and whether MaxiK channel are expressed at mossy fiber axon and   terminals (Cox et al, 2014; PMID:24486049). DRG (L4/L5) from rats was used   for a western blot with this antibody (Zhang et al, 2012; PMID:22607137).

Antibody First Published

Zhang et al. Inflammation-induced   changes in BK(Ca) currents in cutaneous dorsal root ganglion neurons from the   adult rat Mol Pain. 2012 Jul 4;8:37. PMID:22607137

Note on publication

This study provides additional   support for the conclusion that it may be possible, if not necessary, to   selectively treat pain arising from specific body regions.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.