Anti-MMP9 mAbs

Cat. No.

MABL-183

Name

Anti-MMP9  mAbs

Clone No.

AFD-L51/82R

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IF, IHC

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for the MMP protein. MMP9 could play a role in bone osteoclastic resorption. It cleaves type IV  and type V collagen into large C-terminal three quarter fragments and   shorter N-terminal one quarter fragments.

Alternative Name

Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; L51/82

UniProt

P50282

Immunogen

The original version of this antibody was  raised by immunizing a mouse with human MMP-9 residues 107– 445. Then the hybridomas were sequenced by addgene and based on the variable domain   sequences new recombinant formats were created.

Application Notes

Small bowel and colonic muscularis   lysates from C57Bl/6 mice were immunoblotted with this antibody (Moore et al,   2011; PMID:21703213). This antibody was used for immunofluorescence on   postnatal brains of 125 Sprague-Dawley rats (Aujla and Huntley, 2014; PMID:24114974).   Immunoblot was preformed with this antibody on adult rat whole-hippocampal   lysates (Aujla and Huntley, 2014; PMID:24114974). An immunoblot was preformed   on HEK293 cells with this antibody (Duellman et al, 2012; PMID:22942228).   This antibody was used for immunohistochemistry on HEK293 cells (Duellman et   al, 2014; PMID:24627221). A western blot was preformed with this antibody on   HEK293 cells (Duellman et al, 2014; PMID:24627221). Mice brain sections were   used for immunohistochemistry with this antibody (Kuo et al, 2020;   PMID:32926126).

Antibody First Published

Moore et al. Matrix   metalloproteinase-9 inhibition reduces inflammation and improves motility in   murine models of postoperative ileus Gastroenterology. 2011   Oct;141(4):1283-92, 1292.e1-4. PMID:21703213

Note on publication

Mice and rats underwent laparotomy and bowel manipulation; bowel tissues were collected 3 to 24 hours later and analyzed by real-time reverse-transcriptase polymerase chain reaction, immunoblot, in situ zymography, and functional analyses.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.