Key features and details | |
Cat. No. | MABL-184 |
Name | Anti-GABA-A-R-Alpha5 mAbs |
Clone No. | AFD-N415/24R |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for GABA-A-R-Alpha5. It does not cross-react with GABA-A-R-Alpha2. GABA-A-R-Alpha5 is a ligand-gated chloride channel subunit which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. May be involved in GABA-A receptor assembly, and GABA-A receptor immobilization and accumulation by gephyrin at the synapsee. |
Alternative Name | Gamma-aminobutyric acid receptor subunit alpha-5; GABRA5; GABA(A) receptor subunit alpha-5; N415/24 |
UniProt | P31644 |
Immunogen | This antibody was raised by immunizing a mouse with fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA-A-R-Alpha5 receptor subunit alpha-5 and Gabra5. |
Application Notes | This antibody was used for a western blot on mouse cell lysates (Davenport et al, 2020; PMID:33096025). Western blot was preformed on mouse hippocampal protein samples with this antibody (Gruol et al, 2018; PMID:29787738). Lysates from L3 to L5 DRG and the ipsilateral dorsal section of rats were used for western blot with this antibody (Franco-Enzástiga et al, 2020; PMID:32750173).Western blot was preformed on human brain, and on KO mouse brain with this antibody (Gong et al, 2016; PMID:26644354). |
Antibody First Published | Gong et al. Developing high-quality mouse monoclonal antibodies for neuroscience research - approaches, perspectives and opportunities N Biotechnol. 2016 Sep 25;33(5 Pt A):551-64. PMID:26644354 |
Note on publication | A methodology of multi-step mAb screening focused on identifying mAbs exhibiting efficacy and specificity in labeling mammalian brain samples is described. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
