Anti-Kv1.5 K+ channel mAbs

Cat. No.

MABL-211

Name

Anti-Kv1.5 K+ channel  mAbs

Clone No.

AFD-K7/45R

From

Recombinant Antibody

Isotype

Engineer antibody

Application

functional assays, IP, WB, ELISA,   IF, IHC

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for Kv1.5.   This antibody recognized a single protein band at 70 kDa on western blot of   lysates from Kv1.5-transfected COS cells. No band of equivalent size is   observed from rat brain samples and Kv1.5-untransfected COS cells Kv1.5 is a   voltage-gated potassium channel that mediates transmembrane potassium   transport in excitable membranes. Forms tetrameric potassium-selective   channels through which potassium ions pass in accordance with their   electrochemical gradient.

Alternative Name

Potassium voltage-gated channel subfamily A   member 5; Kcna5; RCK7; RK4; Voltage-gated potassium channel subunit Kv1.5;   K7/45

UniProt

P19024

Immunogen

This antibody was raised by   immunizing BALB/c mice with a fusion protein of amino acids 542-602 of rat   Kv1.5.

Application Notes

COS-1 cells were used for   immunofluorescence with this antibody (Bekele-Arcuri et al, 1996;   PMID:8938716). Immunohistochemistry was preformed on rat brains using this   antibody (Bekele- Arcuri et al, 1996; PMID:8938716). GST fusion proteins were   used for an ELISA with this antibody (Bekele- Arcuri et al, 1996;   PMID:8938716). Immunofluorescence was preformed on COS-1 cells using this   antibody (Murakoshi and Trimmer, 1999; PMID:10024359). Proteins from the atrial myocardium and rat brain tissue was immunoprecipitated using this   antibody (Godreau et al, 2002; PMID:12445884). 

Antibody First Published

in: Bekele-Arcuri et al Generation   and characterization of subtype-specific monoclonal antibodies to K+ channel   alpha- and beta-subunit polypeptides Neuropharmacology.1996;35(7):851-65. PMID:8938716

Note on publication

Describes the generation of the anti   Kv1.5 antibody

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.