Key features and details | |
Cat. No. | MABL-212 |
Name | Anti-Kv1.6 K+ channel mAbs |
Clone No. | AFD-K19/36R |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for Kv1.6. Kv1.6 is a voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. |
Alternative Name | Potassium voltage-gated channel subfamily A member 6; Kcna6; RCK2; Voltage-gated potassium channel subunit Kv1.6; Voltage-gated potassium channel subunit Kv2; K19/36 |
UniProt | P17659 |
Immunogen | This antibody was raised by immunizing BALB/c mice with a synthetic peptide 507-525 of rat Kv1.6. |
Application Notes | COS-1 cells were used for immunofluorescence with this antibody (Bekele-Arcuri et al, 1996; PMID:8938716). Immunohistochemistry was preformed on rat brains using this antibody (Bekele- Arcuri et al, 1996; PMID:8938716). GST fusion proteins were used for an ELISA with this antibody (Bekele- Arcuri et al, 1996; PMID:8938716). Immunofluorescence was preformed using this antibody on mouse brain cell lysates (Gazula et al, 2010; PMID:20575068). Western blots of COS cell lysates transfected with rat Kv1.6 were preformed (Gazula et al, 2010; PMID:20575068). Brain cell lysates from 6-month-old C57BL/6 mice were immunoblotted with this antibody (Willis et al, 2018; PMID:29564531). Slice cultures from postnatal day 10–11 Sprague-Dawley rats were used for immunohistochemistry with this antibody (He et al, 2012; PMID:22218089). Both tissue from morton’s neuroma patients and sciatic nerve and DRG from rats was immunoblotted with this antibody (Calvo et al, 2016; PMID:27033551). |
Antibody First Published | Bekele-Arcuri et al Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- andbeta-subunit polypeptides Neuropharmacology.1996;35(7):851-65. PMID:8938716 |
Note on publication | Describes the generation of the anti Kv1.5 antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
