Key features and details | |
Cat. No. | MABL-215 |
Name | Anti-KCNT1/Slo2.2/Slack K+ channel mAbs |
Clone No. | AFD-N3/26R |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for Slo2.2. Slo2.2 is an outwardly rectifying potassium channel subunit that may coassemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro). |
Alternative Name | Potassium channel subfamily T member 1; Kcnt1; Sequence like a calcium-activated potassium channel subunit; N3/26 |
UniProt | |
Immunogen | This antibody was raised against a fusion protein of amino acids 1168-1237 of rat Slo2.2. |
Application Notes | Mouse membrane proteins from whole brain, cerebellum, cortex and spinal cord were immunoprecipitated using this antibody (Martinez-Espinosa et al, 2015; PMID:26559620). Immunoblotting was preformed on mouse membrane proteins from whole brain, cerebellum, cortex and spinal cord using this antibody (Martinez-Espinosa et al, 2015; PMID:26559620). Membrane vesicles from mouse fore- and midbrain were immunoprecipitated using this antibody (Rizzi et al, 2015; PMID:29124216). This antibody was used for immunoblotting on membrane vesicles from mouse fore- and midbrain (Rizzi et al, 2015; PMID:29124216). immunofluorescence was preformed on membrane vesicles from mouse fore- and midbrain using this antibody (Rizzi et al, 2015; PMID:29124216). |
Antibody First Published | Zhang et al. Regulation of Neuronal Excitability by Interaction of Fragile X Mental Retardation Protein with Slack Potassium Channels J Neurosci. 2012 Oct 31;32(44):15318-27.PMID:23115170 |
Note on publication | Aplysia bag cell (BC) neurons, which regulate reproductive behaviors, were used to examine the effects of Slack and FMRP on excitability. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
