Anti-Kv2.1 K+ channel pS603 mAbs

Cat. No.

MABL-216

Name

Anti-Kv2.1 K+ channel pS603 mAbs

Clone No.

AFD-L61/14R

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IF, IHC

Species Reactivity

Rat

Basic Information

Specificity

This antibody is specific for Kv2.1.   Kv2.1 is a voltage-gated potassium channel that mediates transmembrane   potassium transport in excitable membranes, primarily in the brain, but also   in the pancreas and cardiovascular system. Contributes to the regulation of   the action potential (AP) repolarization, duration and frequency of   repetitive AP firing in neurons, muscle cells and endocrine cells and plays a   role in homeostatic attenuation of electrical excitability throughout the   brain.

Alternative Name

Potassium voltage-gated channel subfamily B   member 1; Kcnb1; Delayed rectifier potassium channel 1; DRK1; Voltage-gated   potassium channel subunit Kv2.1

UniProt

Immunogen

This antibody was raised by   immunizing a mouse with the pS603 phosphopeptide.

Application Notes

mmunoblotting was preformed on   Kv2.1-KO and AMIGO-KO mouse brain samples (Mandikian et al, 2014;   PMID:24962901). immunohistochemistry against brain samples from wild-type   mice subjected to CO2 inhalation was preformed with this antibody (Mandikian   et al, 2014; PMID:24962901). This antibody was validated with ELISA on rat   Kv2.1 amino acids 595–616 (Mandikian et al, 2014; PMID:24962901). COS-1 and   CHO cells expressing Kv channels were used for immunofluorescence with this   antibody (Cobb et al, 2015; PMID:26442584). COS-1 and CHO cell lysates   transfected with transfected with rat Kv2.1, Kv1.4, or Kv2.1 were   immunoblotted with this antibody (Cobb et al, 2015; PMID:26442584).

Antibody First Published

Mandikian et al. Cell type-specific spatial   and functional coupling between mammalian brain Kv2.1 K+ channels and   ryanodine receptors J Comp Neurol. 2014 Oct 15;522(15):3555- 74.   PMID:24962901

Note on publication

It was revealed that striatal MSNs   are distinct in their expression of clustered Kv2.1 at plasma membrane sites   juxtaposed to intracellular RyRs, as well as in Kv2.1 phosphorylation state.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.