Key features and details | |
Cat. No. | MABL-229 |
Name | Anti-Nitric Oxide Synthase mAbs |
Clone No. | AFD-3F7-B11-B5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IHC |
Species Reactivity | Bovine, Rat, Human, Mouse |
Basic Information | |
Specificity | The antibody recognizes mouse, rat and bovine NOS. |
Alternative Name | NOS; Nitric oxide synthase; NOS-3F7-B11-B5 |
UniProt | E1BKI3 |
Immunogen | The original antibody was generated by immunizing mice against purified, bovine cerebellum NOS. |
Application Notes | The antibody reacted with bovine cerebellum NOS as well as with structurally different NOSs from cultured bovine aortic endothelial cells and cultured RAW 264.7 macrophages in Western blot analysis. The reactivity of the antibody was confirmed by immunohistochemical staining of rat cerebellum, arterial endothelial cells, and cultured stimulated macrophages. The antibody showed immunoreactivity within the respiratory epithelium but not in the bronchial smooth muscle (Rengasamy et al, 1994; pmid:7528982). |
Antibody First Published | Rengasamy et al. Immunohistochemical demonstration of a paracrine role of nitric oxide in bronchial function. Am J Physiol. 1994 Dec;267(6 Pt 1):L704-11. PMID:7528982 |
Note on publication | The article describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
