Anti-MCSP mAbs

Cat. No.

MABL-230

Name

Anti-MCSP mAbs

Clone No.

AFD-LC007 (M4-3-ML2)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC, IF, IHC

Species Reactivity

Human, Cynomolgus Monkey

Basic Information

Specificity

This antibody recognizes a membrane-proximal epitope on the D3 domain of human MCSP. It also cross reacts with MCSP from cynomolgus monkey.

Alternative Name

CSPG4; Chondroitin sulfate proteoglycan 4;   Melanoma-associated Chondroitin Sulfate Proteoglycan; Melanoma chondroitin sulfate proteoglycan; Chondroitin sulfate proteoglycan NG2

UniProt

Q6UVK1

Immunogen

The original antibody was generated by   immunizing balb/c mice with a synthetic linear peptide corresponding to aa   2177-2221 of the human MCSP, derived from the membrane proximal D3 domain.

Application Notes

The binding characterization of the original  mouse version of this antibody was done using ELISA. This antibody can also binds to native form of MCSP expressed on Colo38 cells in a flow cytometric   analysis. This antibody can also be used for immunofluorescence staining of   MCSP in Colo38 cells. The original mouse version can also detect MCSP   fragments in a western blot (Freytag et al., US9309306). The original mouse  version of this antibody LC007 is capable of inducing antibody dependent   cellular cytotoxicity. This antibody can bind the native epitope on MCSP+   melanoma cells, but there is no induction of internalization. This antibody can be used for immunohistochemical staining of MCSP+ cells in formalin fixed paraffin embedded tissue. 

Antibody First Published

Anti-MCSP antibodies. US9309306

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.