Anti-MAP-2 mAbs

Cat. No.

MABL-232

Name

Anti-MAP-2 mAbs

Clone No.

AFD-AA5 (AP18)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IHC

Species Reactivity

Birds, Clawed frog, Cow, Rat, Human

Basic Information

Specificity

This antibody is specific to the   phosphorylated form of MAP2a, b, c and shows no cross- reactivity with other  MAPs, tau or tubulin. This antibody reacts with dendrites and cell bodies of   neurons. It is useful in studies of neuron structure in normal and malignant brain tissue and in Alzheimer's. MAP-2 cotranslationally removes the   N-terminal methionine from nascent proteins. The N-terminal methionine is  often cleaved when the second residue in the primary sequence is small and uncharged.

Alternative Name

Methionine aminopeptidase 2; MetAP 2; MAP 2; Initiation factor 2- associated 67 kDa glycoprotein; p67; p67eIF2

UniProt

Q3ZC89

Immunogen

The mouse version of this antibody was generated by immunizing mice with taxol-stabalized microtubules from rat brain.

Application Notes

While testing the specificity of the mouse version of this antibody, a western blot on P9 quail and adult rat brain was performed using the mouse version of this antibody. Furthermore, quail   cerebellum was immunologically stained using the mouse version of this antibody (Tucker et al, 1988; pmid:3199190). To examine the relationship   between changes in tau phosphorylation and loss of MAP2 immunmoreactivity,   immunocytochemistry was performed (Ashford et al, 1998; pmid:9786247). While   assessing experience-dependent modifications in MAP2 phosphorylation in rat   olfactory bulb. a western blot was performed against adult rat olfactory bulb  proteins using the mouse version of this antibody.

Antibody First Published

Tucker et al. The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum J Neurosci. 1988 Dec;8(12):4503-12. PMID:3199190

Note on publication

It is shown that avian MAP2 also exists as both high- (Mr approximately   260,000) and low-molecular-weight (Mr approximately 65,000) forms whose   relative abundance changes during brain maturation, indicating a conserved   function for these proteins during vertebrate neuronal morphogenesis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.